pAAV-TRE-NS1-dTomato vector (V015989)

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LOCUS       62056_2525        5842 bp DNA     circular SYN 30-NOV-2021
DEFINITION  Cloning vector pAAV-TRE-NS1-dTomato, complete sequence.
ACCESSION   MZ708021
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W.
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D
  JOURNAL   Nat Methods (2021) In press
  PUBMED    34824475
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Xu W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Neuroscience, UT Southwestern Medical 
            Center, Dallas, TX 75229, USA
REFERENCE   3  (bases 1 to 5842)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Methods
            (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2021) Neuroscience, UT Southwestern Medical Center, Dallas, 
            TX 75229, USA"
FEATURES             Location/Qualifiers
     source          1..5842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    complement(164..245)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(263..964)
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
     CDS             complement(962..982)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     promoter        996..1383
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     CDS             1420..2553
                     /codon_start=1
                     /transl_table=11
                     /product="NS1"
                     /label=NS1
                     /protein_id="UBZ25898.1"
                     /translation="MNMTMSMSMILVGVIMMFLSLGVGADQGCAINFGKRELKCGDGIF
                     IFRDSDDWLNKYSYYPEDPVKLASIVKASFEEGKCGLNSVDSLEHEMWRSRADEINAIF
                     EENEVDISVVVQDPKNVYQRGTHPFSRIRDGLQYGWKTWGKNLVFSPGRKNGSFIIDGK
                     SRKECPFSNRVWNSFQIEEFGTGVFTTRVYMDAVFEYTIDCDGSILGAAVNGKKSAHGS
                     PTFWMGSHEVNGTWMIHTLEALDYKECEWPLTHTIGTSVEESEMFMPRSIGGPVSSHNH
                     IPGYKVQTNGPWMQVPLEVKREACPGTSVIIDGNCDGRGKSTRSTTDSGKVIPEWCCRS
                     CTMPPVSFHGSDGCWYPMEIRPRKTHESHLVRSWVTA"
     polyA_signal    2589..3065
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3105..3245
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3320..3775
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4057..4161
                     /label=AmpR promoter
     CDS             4162..5019
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5193..5781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"