pJDHSP vector (V015979)

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LOCUS       62056_13110        4950 bp DNA     circular SYN 31-MAY-2020
DEFINITION  Expression vector pJDHSP, complete sequence.
ACCESSION   KX267661
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Jia B, Hu Z, Huang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2016) College of Life Sciences, Shenzhen 
            University, Shenzhen Key Laboratory of Marine Bioresource and 
            Eco-Environmental Science, 3688, Nanhai Road, Shenzhen, Guangdong 
            518060, China
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2016) College of Life Sciences, Shenzhen University, 
            Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental 
            Science, 3688, Nanhai Road, Shenzhen, Guangdong 518060, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     381..397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      476..742
                     /note="Chlamydomonas reinhardtii heat shock protein 70A 
                     (HSP70Ap)"
                     /regulatory_class="promoter"
     regulatory      748..972
                     /note="Chlamydomonas reinhardtii ribulose-1,5-bisphosphate 
                     carboxylase/oxygenase small subunit 2 (RBCS2p)"
                     /regulatory_class="promoter"
     misc_feature    970..1126
                     /label=5' sequence of RBCS2 gene containing intron 1
                     /note="5' sequence of RBCS2 gene containing intron 1"
     misc_feature    1127..1168
                     /note="multiple cloning sites in frame with the 5'
                     sequences of RBCS2 gene"
     misc_feature    1200..1452
                     /note="Chlamydomonas reinhardtii ribulose-1,5-bisphosphate 
                     carboxylase/oxygenase small subunit 2 3' UTR"
     misc_feature    1475..2611
                     /label=zeocin resistance gene expression cassette
                     /note="zeocin resistance gene expression cassette"
     primer_bind     complement(2729..2745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2753..2769)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2777..2807)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2822..2843)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3131..3719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3893..4750)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4751..4855)
                     /label=AmpR promoter