pCJH5_pDEST17-EcG3PD vector (V015977)

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LOCUS       V015977                 5734 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V015977
VERSION     V015977
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..5734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             113..130
                     /label="6xHis"
                     /note="6xHis affinity tag"
     protein_bind    140..164
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             170..1186
                     /gene="gpsA"
                     /label="Glycerol-3-phosphate dehydrogenase [NAD(P)+]"
                     /note="Glycerol-3-phosphate dehydrogenase [NAD(P)+] from
                     Escherichia coli (strain SMS-3-5 / SECEC). Accession#:
                     B1LK47"
     protein_bind    complement(1200..1224)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1291..1338
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(1704..1732)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        1845..1949
                     /label="AmpR promoter"
     CDS             1950..2807
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2981..3569
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3755..3897)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4002..4190)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"