pYQS040 vector (V015971)

LOCUS       62056_23185        9897 bp DNA     circular SYN 19-JUL-2021
DEFINITION  Biosensor vector pYQS040, complete sequence.
ACCESSION   MW810319
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Sekurova ON, Sun YQ, Zehl M, Ruckert C, Stich A, Busche T, 
            Kalinowski J, Zotchev SB.
  TITLE     Coupling of the engineered DNA 'mutator' to a biosensor as a new 
            paradigm for activation of silent biosynthetic gene clusters in 
            Streptomyces
  JOURNAL   Nucleic Acids Res. (2021) In press
  PUBMED    34197612
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Sekurova ON, Sun Y-Q., Zehl M, Ruckert C, Stich A, Busche T, 
            Kalinowski J, Zotchev SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2021) CeBiTec, Bielefeld University, 
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
REFERENCE   3  (bases 1 to 9897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2021) CeBiTec, Bielefeld University, Universitaetsstr. 27, 
            Bielefeld, NRW 33615, Germany"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: canu v. 2.1.1
            Sequencing Technology :: Illumina; ONT
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /note="attP; phiC31 attachment site"
     CDS             83..1897
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
     primer_bind     2343..2359
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2517..2552
                     /regulatory_class="promoter"
     misc_feature    2553
                     /label=transcription start site
                     /note="transcription start site"
     gene            2659..6510
                     /gene="bpsA"
                     /label=bpsA
     CDS             2659..6510
                     /codon_start=1
                     /transl_table=11
                     /gene="bpsA"
                     /product="indigoidine synthase"
                     /label=bpsA
                     /protein_id="QXN60064.1"
                     /translation="MTLQETSVLEPTLRGTTTLPDLLAKRVAEHPEATAVAYRDEKLTY
                     RELASRSSALAEYLRHLGVSTDDCVGLFVEPSIDLMVGAWGILSAGAAYLPLSPEYPED
                     RLRYMIENSQAKIILAQQRLVTRLRELAPQDVRVVTLRESEAFVLPEGQVAPAIEGARP
                     DSLAYVIYTSGSTGKPKGVMIEHHSIVSQLGWLRETYGIDRSKTILQKTPMSFDAAQWE
                     ILSPANGATVVMGAPGVYADPEGLIETIVKYGVTTLQCVPTLLQGLLDTEKFPECTSLQ
                     QIFSGGEALSRLLAIQTTQEMPGRALINVYGPTECTINSSSYAVDPAELGEAPQSISIG
                     APVADTEYHILGKEDLKPVGVGEIGELYIGGGQLARGYLHRPDLTAERFLEIEVTEGAG
                     PVRLYKTGDLGQWNPDGTVQFAGRADNQVKLRGYRVELDEISLAIENHDWVRNAAVIVK
                     NDGRTGFQNLIACVELSEKEAALMDQGNHGSHHASKKSKLQVKAQLSNPGLRDDADLAA
                     RVAYDLPGAEPTPEQRSRVFARKTYRFYEGGAVTEADLLALLGGQVPAAYSRKAADLAP
                     AELGQILRWFGQYLSEERLLPKYGYASPGALYATQLYFELEGVGGLQPGYYYYQPQRHQ
                     LVLISEKAATGRPTAHIHFIGKRGGIEPVYKNNIQEVLEIETGHIVGLFEQVLPAYGLD
                     IRDLAYEPAVRDLLDVPEEDFYLGTFELVPHTGRREDHAEVYVQTHGSKVANLPEGQYR
                     YADGTLTRFSDDIVLKKQVIAINQSVYQAASFGISVISRAPEEWMHYVTLGKKLQHLMM
                     NGLGLGFMSSGYSSKTGNPLPASRRIDSVLQANGVESGPSYFFVGGRVSDEQLGHEGMR
                     EDSVHMRGPAELIRDDLVSFLPDYMIPNRVVVFERLPLSANGKIDAKALAASDQVNAEL
                     VERPFVAPRTETEKEIAEVWAKSLRRESVSVQDDFFESGGNSLIAVGLIRELNSRLGVS
                     LPLQSVLESPTVEKLSRRLEREVAQESSRLVRLHAETGKDRPVLCWPGLGGYPMNLRTL
                     AGEIGLGRSFYGIQAHGINEGEAPYATITEMAKADIEAIKELQPKGPYTLWGYSFGARV
                     AFETAYQLEQAGEKVDNLFLIAPGSPTVRAENGKVYGREASFANRAYTTILFSVFTGTI
                     SGPDLEKCLESATDEESFAGFISELKGIDVDLAKRIISVVGQTYEFEYSFRELAERTLA
                     APVTIFKARGDDYSFIENSNGYSAEPPTVIDLDADHYSLLRTPDIGELVKHIRYLLGE"
     regulatory      6526..6573
                     /regulatory_class="terminator"
     primer_bind     complement(6607..6623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6631..6647)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6655..6685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6700..6721)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7009..7597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             7801..8601
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(9038..9406)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(9439..9548)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(9786..9797)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    9868..9897
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"