pUC18T-mini-Tn7T-Apr-mCherry vector (V015964)

LOCUS       V015964                 6222 bp    DNA     circular SYN 02-APR-2019
DEFINITION  Exported.
ACCESSION   V015964
VERSION     V015964
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Crisostomo L, Fernando DM, Chao TC,
            Pelka P, Schweizer HP, Kumar A.
  TITLE     Next Generation of Tn7-based single copy insertion elements for use
            in multi- and pandrug resistant strains of Acinetobacter baumannii
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
   PUBMED   30902859
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Department of Microbiology, University of
            Manitoba, 45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada
REFERENCE   3  (bases 1 to 6222)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-SEP-2018) Department of Microbiology, University of Manitoba,
            45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada"
FEATURES             Location/Qualifiers
     source          1..6222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label="n7R"
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      629..723
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1123..1923)
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     promoter        complement(2164..2192)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     misc_feature    2233..2280
                     /note="FRT; Flp recombinase target site"
     misc_feature    2307..2355
                     /note="MCS; multiple cloning site"
     CDS             complement(2370..3077)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             complement(3389..3502)
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     misc_feature    3718..3730
                     /note="MCS; multiple cloning site"
     mobile_element  complement(3739..3904)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(4063..4171)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      complement(4403..4991)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5165..6022)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6023..6127)
                     /label="AmpR promoter"