Basic Vector Information
- Vector Name:
- AGG1879-U6-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2809 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J
AGG1879-U6-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AGG1879-U6-1 vector Sequence
LOCUS 62056_346 2809 bp DNA circular SYN 12-AUG-2020 DEFINITION Cloning vector AGG1879:U6-1, complete sequence. ACCESSION MT119960 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2809) AUTHORS Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, Leftwich PT, Fragkoudis R, Noad R, Alphey L. TITLE Cas13b-dependent and Cas13b-independent RNA knockdown of viral sequences in mosquito cells following guide RNA expression JOURNAL Commun Biol 3 (1), 413 (2020) PUBMED 32737398 REFERENCE 2 (bases 1 to 2809) AUTHORS Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, Leftwich PT, Fragkoudis R, Noad R, Alphey L. TITLE Direct Submission JOURNAL Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK REFERENCE 3 (bases 1 to 2809) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Commun Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK" FEATURES Location/Qualifiers source 1..2809 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 531..1119 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 1410..1431 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2204..2308 /label=AmpR promoter CDS join(2309..2809,1..357) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW"
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