pTet-33tev vector (V015935)

LOCUS       62056_21155        3311 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pTet-33tev, complete sequence.
ACCESSION   MK238517
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-NOV-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     regulatory      713..723
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     CDS             733..1434
                     /label=TEV protease
                     /note="tobacco etch virus protease (Kapust et al., 2001)"
     terminator      1476..1547
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1563..1590
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1752..2297)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2411..2505)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2529..3185)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3186..3288)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"