pCHX50 vector (V015925)

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LOCUS       62056_6305        7632 bp DNA     circular SYN 15-MAY-2019
DEFINITION  Cloning vector pCHX50, complete sequence.
ACCESSION   MK448012
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Chassin H, Muller M, Tigges M, Scheller L, Lang M, Fussenegger M.
  TITLE     A modular degron library for synthetic circuits in mammalian cells
  JOURNAL   Nat Commun 10 (1), 2013 (2019)
  PUBMED    31043592
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Chassin H, Mueller M, Tigges M, Scheller L, Lang M, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 
            4058, Switzerland
REFERENCE   3  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "2013"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, 
            Switzerland"
FEATURES             Location/Qualifiers
     source          1..7632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        40..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        420..623
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    727..954
                     /label=UbVR
                     /note="UbVR"
     misc_feature    967..1194
                     /label=UbVR
                     /note="UbVR"
     misc_feature    1207..1434
                     /label=UbVR
                     /note="UbVR"
     CDS             1447..2448
                     /label=tTA
                     /note="tetracycline-controlled transactivator, comprising a
                     fusion of the tetracycline repressor TetR with the 
                     C-terminal activation domain of herpes simplex virus VP16"
     CDS             2461..3147
                     /label=Dendra2
                     /note="monomeric photoswitchable green-to-red fluorescent 
                     protein from Dendronephthya"
     misc_feature    3179..3191
                     /label=Activation Domain
                     /note="Activation Domain"
     misc_feature    3185..3191
                     /label=BB2
                     /note="BB2"
     polyA_signal    3232..3456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3502..3930
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3944..4273
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4340..5131
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5308..5441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5478..5494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5502..5518)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5526..5556)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5571..5592)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5880..6465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6639..7496)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7497..7601)
                     /label=AmpR promoter