pPtPDS1-M1 vector (V015905)

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LOCUS       62056_18625        6346 bp DNA     circular SYN 12-JUN-2019
DEFINITION  Transformation vector pPtPDS1-M1, complete sequence.
ACCESSION   MK645852
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Taparia Y, Zarka A, Leu S, Zarivach R, Boussiba S, Khozin-Goldberg 
            I.
  TITLE     A novel endogenous selection marker for the diatom Phaeodactylum 
            tricornutum based on a unique mutation in phytoene desaturase 1
  JOURNAL   Sci Rep 9 (1), 8217 (2019)
  PUBMED    31160749
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Taparia Y, Leu S, Zarka A, Zarivach R, Khozin-Goldberg I, Boussiba 
            S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2019) Albert Katz International School for Desert 
            Research, Ben-Gurion University of The Negev, French Associates 
            Institute for Agriculture and Biotechnology, Sede Boqer, Israel 
            8499000, Israel
REFERENCE   3  (bases 1 to 6346)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "8217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAR-2019) Albert Katz International School for Desert Research, 
            Ben-Gurion University of The Negev, French Associates Institute for 
            Agriculture and Biotechnology, Sede Boqer, Israel 8499000, Israel"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..453
                     /regulatory_class="promoter"
     CDS             480..1196
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1213..1269
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      1270..1598
                     /regulatory_class="terminator"
     regulatory      1605..1850
                     /regulatory_class="promoter"
     CDS             2117..2131
                     /label=SNAC tag
                     /note="tag for protein cleavage using Ni2+ ion (Dang et
                     al., 2019)"
     regulatory      3726..3967
                     /regulatory_class="terminator"
     promoter        complement(3988..4006)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4264..4852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5026..5883)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5884..5988)
                     /label=AmpR promoter