pCW702-E6E7 vector (V015901)

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LOCUS       V015901                 9540 bp    DNA     circular SYN 06-JUL-2021
DEFINITION  Exported.
ACCESSION   V015901
VERSION     V015901
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Wang C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2020) Sichuan University, West China School of
            Public Health, Renmin Nan Road, Chengdu, Sichuan 610041, China
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2020) Sichuan University, West China School of Public
            Health, Renmin Nan Road, Chengdu, Sichuan 610041, China"
FEATURES             Location/Qualifiers
     source          1..9540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     primer_bind     complement(700..722)
                     /label="pGEX_3 primer"
                     /note="pGEX_3 primer"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             2908..3966
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase"
                     /label="zinc metalloproteinase"
                     /protein_id="QXF31294.1"
                     /translation="MTLTYKNRRITRPFRLQEFPAWSHTTVGGLPITEWISEDEQGAMD
                     TIFVSVRDAAYEIINKKGATFYGVAAALARITKAILNNENAILPLSVYLDGHYGMNDIY
                     IGAPAVVNRQGVRHIVEMNLNDKEKEQMKNSADTLKKVLDKGGEIDSFVHYGLNCNNAF
                     WDGQEILYGDGDKKNFKPFSCAKTIVGHELTHAVIQYSAGLEYEGQSGALNESFADVFG
                     YFIAPNHWLIGEDVCVRGSRDGRIRSIKDPDKYNQAAHMKDYESLPITEEGDWGGVHYN
                     SGIPNKAAYNTITKLGKEKTEQRRFRALKYYLTKKAQFTDAKKALQQAAKDLYGEDASK
                     KVAEAWEAVGVN"
     CDS             4344..4370
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5334..5366
                     /label="VSV-G tag"
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
     CDS             complement(5783..6514)
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /label="DNA alkylation repair protein"
                     /protein_id="QXF31296.1"
                     /translation="MRLRMLFLLYLLYSRGVTRMITFDQLDTELQALENPNTIKIFRNH
                     GCPDSLDLYGLKIGDLKKIIRREKLTKNHELAVKLIESNNSDLIYLGLLAINPNKITTE
                     QIEKWNIAFRETWSQLTFGLASIVSKRDDALLFAKTWIESDYDLTKSMGWQIYSEHINN
                     LPEAETLLQRAKETLQTETNRTRYSMNGFIITCGIYKDDLHEKAMEAAKSVGKVHVNLG
                     NTACKVPDAISYIEKARNRTK"
     CDS             7220..7951
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"