pETcc2-TkGlpKMsAK_1 vector (V015888)

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LOCUS       V015888                 5197 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V015888
VERSION     V015888
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Coia G, Nebl T, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5197)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..5197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1492
                     /gene="glpK"
                     /label="Glycerol kinase"
                     /note="Glycerol kinase from Thermococcus kodakarensis
                     (strain ATCC BAA-918 / JCM 12380 / KOD1). Accession#:
                     O93623"
     CDS             1550..2677
                     /gene="ackA"
                     /label="Acetate kinase"
                     /note="Acetate kinase from Mycolicibacterium smegmatis
                     (strain ATCC 700084 / mc(2)155). Accession#: A0QQK1"
     terminator      2702..2749
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(3115..3143)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        3256..3360
                     /label="AmpR promoter"
     CDS             3361..4218
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4392..4980
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5060..5078
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             5110..5132
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5151..5168
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             5178..5195
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"