pAS59_yPSAT1_n vector (V015887)

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LOCUS       V015887                 5775 bp    DNA     circular SYN 01-MAR-2020
DEFINITION  Exported.
ACCESSION   V015887
VERSION     V015887
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Cromie G.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA
            98027, USA
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 98027,
            USA"
FEATURES             Location/Qualifiers
     source          1..5775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(719..823)
                     /label="AmpR promoter"
     primer_bind     1297..1313
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1421..2530
                     /gene="PSAT1"
                     /label="Phosphoserine aminotransferase"
                     /note="Phosphoserine aminotransferase from Homo sapiens.
                     Accession#: Q9Y617"
     gene            3087..4206
                     /label="natMX6"
                     /note="yeast selectable marker conferring nourseothricin
                     resistance"
     primer_bind     complement(4472..4488)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4496..4512)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4520..4550)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4565..4586)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4874..5462)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(5636..5775,1..718))
                     /label="AmpR"
                     /note="beta-lactamase"