pET28AMP_MaIE_AGF30 vector (V015885)

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LOCUS       62056_10170        7000 bp DNA     circular SYN 09-OCT-2019
DEFINITION  Cloning vector pET28AMP_MaIE_AGF30, complete sequence.
ACCESSION   MK659889
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Faculty of Chemistry, University of Gdansk, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2019) Faculty of Chemistry, University of Gdansk, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(13..30)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(121..143)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(158..182)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        510..587
                     /label=lacI promoter
     CDS             588..1667
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1683..1704
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             2479..2667
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2772..2914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3100..3688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3810..4622
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(4718..5173)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(5210..5257)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5324..5341)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    complement(5375..5914)
                     /label=AGF_10
                     /note="AGF_10"
     misc_feature    complement(5918..6457)
                     /label=AGF_10
                     /note="AGF_10"
     misc_feature    complement(6461..7000)
                     /label=AGF_10
                     /note="AGF_10"