Basic Vector Information
- Vector Name:
- pBluescript-II-KS(+)-EACMCV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7273 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Hoyer JS, Fondong VN, Dallas MM, Aimone CD
pBluescript-II-KS(+)-EACMCV vector Vector Map
pBluescript-II-KS(+)-EACMCV vector Sequence
LOCUS 62056_4120 7273 bp DNA circular SYN 18-NOV-2020
DEFINITION Cloning vector pBluescript II KS(+) EACMCV DNA-A 1.6mer, complete
sequence.
ACCESSION MT856195
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7273)
AUTHORS Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S,
Hanley-Bowdoin L.
TITLE Deeply Sequenced Infectious Clones of Key Cassava Begomovirus
Isolates from Cameroon
JOURNAL Microbiol Resour Announc 9 (46), e00802-20 (2020)
PUBMED 33184153
REFERENCE 2 (bases 1 to 7273)
AUTHORS Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S,
Hanley-Bowdoin L.
TITLE Direct Submission
JOURNAL Submitted (06-AUG-2020) Ecology, Evolution, and Natural Resources,
Rutgers University, 14 College Farm Rd, New Brunswick, NJ 08901, USA
REFERENCE 3 (bases 1 to 7273)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol
Resour Announc"; date: "2020"; volume: "9"; issue: "46"; pages:
"e00802-20"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-AUG-2020) Ecology, Evolution, and Natural Resources, Rutgers
University, 14 College Farm Rd, New Brunswick, NJ 08901, USA"
COMMENT ##Assembly-Data-START##
Assembly Method :: Benchling v. 2019-01-22
Sequencing Technology :: Illumina; Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7273
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(4..861)
/label=AmpR
/note="beta-lactamase"
promoter complement(862..966)
/label=AmpR promoter
rep_origin complement(992..1447)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 1589..1605
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 1615..1633
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 1666..1682
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 2299..5098
/label=EACMCV DNA-A monomer unit, nick site to nick site
/note="EACMCV DNA-A monomer unit, nick site to nick site"
gene 2471..2818
/gene="AV2"
/label=AV2
CDS 2471..2818
/codon_start=1
/transl_table=11
/gene="AV2"
/product="AV2"
/label=AV2
/protein_id="QNR00655.1"
/translation="MWNPLVNDFPETVHGFRSMLAVKYLLHLEQEYDRGTVGAEYIRDL
IGVLRCKSYGEATRRYNNLNTRIQGAEEAELRQPIHEPCGCPYCPRHQKQNMGQQAHVS
EAQDVQDVSKP"
gene 2631..3404
/gene="AV1"
/label=AV1
CDS 2631..3404
/codon_start=1
/transl_table=11
/gene="AV1"
/product="CP"
/label=AV1
/protein_id="QNR00654.1"
/translation="MAKRPGDIIISTPVSKVRRRLNFDSPYTNRVVAPTVRVTRSKIWA
NRPMYRKPKMYRMYRSPDVPKGCEGPCKVQSYEQRDDVKHTGIVRCVSEVTRGSGITHR
VGKRLCVKSIYILGKIWMDENIKKQNHTNHVMFFLVRDRRPYGPSPQDFGQVFNMFDNE
PTTATVKNDYRDRFQVLRKFHATVVGGPSGMKEQALVKRFFRINNHIVYNHQEQAKYEN
HTENALLLYMACTHASNPVYATLKIRIYFYDAVTN"
misc_feature 3226..4477
/note="unique part of EACMCV DNA-A partial tandem dimer,
HindIII to BamHI, noninclusive"
gene complement(3401..3805)
/gene="AC3"
/label=AC3
CDS complement(3401..3805)
/codon_start=1
/transl_table=11
/gene="AC3"
/product="REn"
/label=AC3
/protein_id="QNR00659.1"
/translation="MDLRTGEDIGALQWRSGAFIWEIPNPLYFTILNHDSMPFNMTHDI
IDVQIRFNYNLRQALGMHKCFLNFRIWTRLHPQTWRFFRTFRTQVMKYLTNLGVISIST
VIDAVHHVLNVVFVGTLSVSQDHAIKFNIY"
gene complement(3546..3953)
/gene="AC2"
/label=AC2
CDS complement(3546..3953)
/codon_start=1
/transl_table=11
/gene="AC2"
/product="TrAP"
/label=AC2
/protein_id="QNR00658.1"
/translation="MRSSSPSQSHSSPPPIKARHRQAKLRAPRRRRIDLTCGCSIYRSI
NCHNHGFTHRGRHWCSSMEEWRLYLGDSKSPIFHNPQPRQHAVQHDSRHNRCPDSVQLQ
PEASTGDAQVFSQLPDLDSFTSSDLAFLQNL"
gene complement(3883..4941)
/gene="AC1"
/label=AC1
CDS complement(3883..4941)
/codon_start=1
/transl_table=11
/gene="AC1"
/product="Rep"
/label=AC1
/protein_id="QNR00656.1"
/translation="MPRAGRFQINAKNYFITYPRCSLAKEEALSQLKAISYPTNIKFIR
VCRELHQDGVPHLHVLIQFEGKFQCTNPRFFDLISPSRSTHFHPNIQGAKSSSDVKAYI
EKGGEFLDDGIFQVDARSARGEGQHLAQVYAEALNASSKSEALQIIKEKDPKSFFLQFH
NISANADRIFQAPPQTYVSPFLSSSFTQVPEEIEVWVSENICRPAARPWRPISIVLEGD
SRTGKTMWARSLGPHNYLCGHLDLSPKIYSNDAWYNVIDDVDPHYLKHFKEFMGAQRDW
QSNTKYGKPIQIKGGIPTIFLCNPGPNSSYKEYLDEDKNSNLKNWAIKNALFISLTEPL
FSSTDQSQAQAS"
misc_feature 4478..6025
/note="duplicated part of EACMCV DNA-A partial tandem
dimer, BamHI to HindIII, inclusive, copy 2"
gene complement(4551..4784)
/gene="AC4"
/label=AC4
CDS complement(4551..4784)
/codon_start=1
/transl_table=11
/gene="AC4"
/product="AC4"
/label=AC4
/protein_id="QNR00657.1"
/translation="MGCLISMFSSNSKASSNVPTPDSSISFPHPDQHISIRTFRELNHR
PMSRLTLKREGNFLTMEFSKSMPEVPGGRASI"
primer_bind complement(6028..6044)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(6074..6092)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6113..6129)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6137..6153)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6161..6191)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6206..6227)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6515..7103)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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