pKK89 vector (V015871)

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LOCUS       62056_13485        4533 bp DNA     circular SYN 15-FEB-2020
DEFINITION  Expression vector pKK89, complete sequence.
ACCESSION   MN811113
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Krawczyk K, Scheller L, Kim H, Fussenegger M.
  TITLE     Rewiring of endogenous signaling pathways to genomic targets for 
            therapeutic cell reprogramming
  JOURNAL   Nat Commun 11 (1), 608 (2020)
  PUBMED    32001704
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Krawczyk KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 
            4058, Switzerland
REFERENCE   3  (bases 1 to 4533)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, 
            Switzerland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(178..766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(940..1797)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1798..1902)
                     /label=AmpR promoter
     rep_origin      1929..2384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    2515..2563
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    2577..2668
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        2846..2877
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    2916..2966
                     /gene="NanoLuc-Fc"
                     /label=Gaussia luciferase signal peptide
                     /note="Gaussia luciferase signal peptide"
     CDS             2967..3476
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     misc_feature    3477..3497
                     /gene="NanoLuc-Fc"
                     /label=flexible linker
                     /note="flexible linker"
     misc_feature    3498..4160
                     /gene="NanoLuc-Fc"
                     /label=murine Fc
                     /note="murine Fc"
     polyA_signal    complement(4189..4310)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"