pYI37 vector (V015868)

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LOCUS       V015868                 8020 bp    DNA     circular SYN 04-AUG-2020
DEFINITION  Exported.
ACCESSION   V015868
VERSION     V015868
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Inaba Y, West AC, Banta S.
  TITLE     Enhanced microbial corrosion of stainless steel by Acidithiobacillus
            ferrooxidans through the manipulation of substrate oxidation and
            overexpression of rus
  JOURNAL   Biotechnol. Bioeng. (2020) In press
   PUBMED   32687219
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Inaba Y, West AC, Banta S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2020) Chemical Engineering, Columbia University,
            500 W 120th St., New York, NY 10025, USA
REFERENCE   3  (bases 1 to 8020)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUN-2020) Chemical Engineering, Columbia University, 500 W 120th
            St., New York, NY 10025, USA"
FEATURES             Location/Qualifiers
     source          1..8020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(329..1120)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        1664..1692
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1700..1716
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1741..2301
                     /gene="rus"
                     /label="Rusticyanin"
                     /note="Rusticyanin from Acidithiobacillus ferrooxidans
                     (strain ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455).
                     Accession#: B7JAQ0"
     CDS             2308..2325
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2329..2415
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2507..2534
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(2603..2997)
                     /direction=LEFT
                     /label="RSF1010 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     RSF1010; requires the RSF1010 RepA/B/C proteins for
                     replication (Scholz et al., 1989)"
     oriT            3339..3426
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     CDS             4665..5633
                     /label="RSF1010 RepB"
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             6147..6983
                     /label="RSF1010 RepA"
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             6973..7821
                     /label="RSF1010 RepC"
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"