pJC01 vector (V015867)

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LOCUS       V015867                 7711 bp    DNA     circular SYN 10-MAR-2019
DEFINITION  Exported.
ACCESSION   V015867
VERSION     V015867
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Cui J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) MCB, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-JUN-2018) MCB, Dartmouth College, 14 Engineering Drive, Hanover,
            NH 03755, USA"
FEATURES             Location/Qualifiers
     source          1..7711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..1047)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(1095..1317)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     misc_feature    1844..2366
                     /label="upstream homology recombination flank"
                     /note="upstream homology recombination flank"
     gene            2367..3212
                     /gene="nfnA"
                     /label="nfnA"
     CDS             2367..3212
                     /codon_start=1
                     /transl_table=11
                     /gene="nfnA"
                     /product="NADH-dependent reduced ferredoxin:NADP
                     oxidoreductase subunit alpha"
                     /EC_number="1.6.1.4"
                     /label="nfnA"
                     /note="Clo1313_1848"
                     /protein_id="QBI89790.1"
                     /translation="MFKIVKKKILNPQVKLMEISAPHVAKKAEPGQFIILRVNENGERI
                     PLTIADFDREKGTVTIIFQEVGKTTKLLGTLEEGDELLDFVGPLGKASHFENVKKAAVI
                     GGGLGTAIAYPQAKKLHSMGVEVHTIAGFRNKDLIILEDEMRAVSSKLFITTDDGSNGN
                     KGFVSDVLKKLIEEGNKYDLVVAIGPLIMMKVISELTRPYGIKTIVSMNPVMIDGTGMC
                     GGCRVTVGGEIKFACVDGPDFDGHLVDFDEAMRRQAMYKKEESLALEKHNCKLGVEKNA
                     "
     gene            3205..4599
                     /gene="nfnB"
                     /label="nfnB"
     CDS             3205..4599
                     /codon_start=1
                     /transl_table=11
                     /gene="nfnB"
                     /product="NADH-dependent reduced ferredoxin:NADP
                     oxidoreductase subunit beta"
                     /EC_number="1.6.1.4"
                     /label="nfnB"
                     /note="Clo1313_1849"
                     /protein_id="QBI89791.1"
                     /translation="MPNMSPKKVPMPEQDPNVRIKNFLEVALGYTEQMAMEEAQRCLNC
                     KHKPCVSGCPVNVKIPEFVQLIAQGKFEKAYNKIRETNNLPAICGRVCPQENQCEKFCV
                     RGIKGEPVAIGRLERFAADWHMKNGTTSYEKPEKNGKRVAVIGSGPASLTCASDLAKLG
                     YEVTIFEAFHVPGGVLMYGIPEFRLPKKLVQEEIETIKQLGVEIKTNMVIGKVYSIDEL
                     KAEGYDAIFIGSGAGLPSFMKIPGENLNGVYSANEFLTRINLMKAYEFPNCDTPVKVGK
                     NVAVVGGGNVAMDAARSAKRLGAENVYIVYRRSEAEMPARLEEIHHAKEEGILFKFLTN
                     PTRILGTDDGWVKGMECIEMELGEPDESGRRRPVPKPGSEHVIDVETVIIAIGQTPNPL
                     IASTTPGLATQKWGGIIVDENTGATNIEGVYAGGDAVTGAATVILAMGAGKKAAKAIDE
                     YLKNKK"
     misc_feature    4600..5164
                     /label="downstream homology recombination flank"
                     /note="downstream homology recombination flank"
     regulatory      5169..5699
                     /label="kanamycin promoter"
                     /note="kanamycin promoter"
                     /regulatory_class="promoter"
     gene            5700..6461
                     /gene="htk"
                     /label="htk"
     CDS             5700..6461
                     /codon_start=1
                     /transl_table=11
                     /gene="htk"
                     /product="highly thermostable kanamycin
                     nucleotidyltransferase"
                     /EC_number="2.7.7.46"
                     /label="htk"
                     /protein_id="QBI89792.1"
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGRFF
                     SILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSDPEK
                     LLESLENFWNGVQEWAERHGYIVDVSKRIPF"
     CDS             6485..7063
                     /gene="tdk"
                     /label="Thymidine kinase"
                     /note="Thymidine kinase from Thermoanaerobacter
                     pseudethanolicus (strain ATCC 33223 / 39E). Accession#:
                     B0K7G9"
     misc_feature    7158..7711
                     /label="gene target internal homology recombination flank"
                     /note="gene target internal homology recombination flank"