3xP3_attB_eGFP_Vasa-AcrIIA4 vector (V015866)

LOCUS       62056_141        7475 bp DNA     circular SYN 14-JUN-2021
DEFINITION  Cloning vector 3xP3_attB_eGFP_Vasa-AcrIIA4, complete sequence.
ACCESSION   MZ172909
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Taxiarchi C, Beaghton A, Illansinhage Don N, Kyrou K, Gribble M, 
            Shittu D, Collins SP, Beisel CL, Galizi R, Crisanti A.
  TITLE     A genetically encoded anti-CRISPR protein constrains gene drive 
            spread and prevents population suppression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Taxiarchi C, Beaghton A, Illansinhage Don N, Kyrou K, Gribble M, 
            Shittu D, Collins SP, Beisel CL, Galizi R, Crisanti A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Life Sciences, Imperial College London, 
            Exhibition Road, London SW7 2AZ, UK
REFERENCE   3  (bases 1 to 7475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2021) Life Sciences, Imperial College London, Exhibition 
            Road, London SW7 2AZ, UK"
FEATURES             Location/Qualifiers
     source          1..7475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      869..1129
                     /label=3xp3
                     /note="3xp3"
                     /regulatory_class="promoter"
     protein_bind    1135..1168
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     CDS             1190..1906
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2032..2153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2540..2644
                     /label=AmpR promoter
     CDS             2645..3502
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3676..4264
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4552..4573
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4588..4618
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4626..4642
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4650..4666
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4724..7014
                     /label=vasa2
                     /note="vasa2"
                     /regulatory_class="promoter"
     CDS             7024..7044
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"