6-AXIG-WUS2-IN2 vector (V015861)

LOCUS       62056_181        5611 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 6-AXIG:WUS2:IN2, complete sequence.
ACCESSION   MN294718
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(274..301)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(393..479)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    519..614
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      663..1973
                     /regulatory_class="promoter"
     CDS             1975..2883
                     /codon_start=1
                     /transl_table=11
                     /product="ZM-WUS2"
                     /label=ZM-WUS2
                     /protein_id="QIN53505.1"
                     /translation="MAANAGGGGAGGGSGSGSVAAPAVCRPSGSRWTPTPEQIRMLKEL
                     YYGCGIRSPSSEQIQRITAMLRQHGKIEGKNVFYWFQNHKARERQKRRLTSLDVNVPAA
                     GAADATTSQLGVLSLSSPPPSGAAPPSPTLGFYAAGNGGGSAVLLDTSSDWGSSGAAMA
                     TETCFLQDYMGVTDTGSSSQWPRFSSSDTIMAAAAARAATTRAPETLPLFPTCGDDGGS
                     GSSSYLPFWGAASTTAGATSSVAIQQQHQLQEQYSFYSNSNSTQLAGTGNQDVSATAAA
                     AAALELSLSSWCSPYPAAGSM"
     regulatory      2959..3453
                     /regulatory_class="terminator"
     primer_bind     complement(3608..3624)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3662..3786
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3859..3877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3882..3898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4011..4817
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4967..5555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"