pVG362_Aste-U6a-Swap3-gRNA vector (V015858)

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LOCUS       62056_22570        4861 bp DNA     circular SYN 07-OCT-2020
DEFINITION  Cloning vector pVG362_Aste-U6a-Swap3-gRNA, complete sequence.
ACCESSION   MW030449
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Adolfi A, Gantz V, Jasinskiene N, Lee H-F., Hwang K, Terradas G, 
            Bulger E, Ramaiah A, Bennett J, Emerson JJ, Marshall J, Bier E.
  TITLE     Efficient population modification gene-drive rescue system in the 
            malaria mosquito Anopheles stephensi
  JOURNAL   Nat Commun (2020) In press
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Adolfi A, Gantz V, Jasinskiene N, Lee H-F., Hwang K, Terradas G, 
            Bulger E, Ramaiah A, Bennett J, Emerson JJ, Marshall J, Bier E.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Developmental and Stem Cell Biology, 
            University of California, San Francisco, 1650 Owens St., San 
            Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2020) Developmental and Stem Cell Biology, University of 
            California, San Francisco, 1650 Owens St., San Francisco, CA 94158, 
            USA"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(944..1801)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(1822..2613)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4065..4084
                     /label=Swap3 Target
                     /note="Swap3 Target"
     misc_RNA        4085..4160
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(4641..4657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4665..4681)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4689..4719)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4734..4755)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."