pTY1-lacI vector (V015820)

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LOCUS       62056_21685        5661 bp DNA     circular SYN 27-DEC-2020
DEFINITION  Cloning vector pTY1-lacI, complete sequence.
ACCESSION   MT702633
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Tack DS, Tonner PD, Pressman A, Olson ND, Levy SF, Romantseva EF, 
            Alperovich N, Vasilyeva O, Ross D.
  TITLE     The genotype-phenotype landscape of an allosteric protein
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Tack DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2020) Cellular Engineering, National Institute of 
            Standards and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD 
            20899, USA
REFERENCE   3  (bases 1 to 5661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUL-2020) Cellular Engineering, National Institute of Standards 
            and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD 20899, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             101..913
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1005..1550
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1904..1951)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      complement(2126..2242)
                     /label=araC terminator
                     /note="araC terminator"
                     /regulatory_class="terminator"
     CDS             complement(2267..3346)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(3375..3428)
                     /label=Plac promoter
                     /note="Plac promoter"
                     /regulatory_class="promoter"
     promoter        3503..3531
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3539..3555
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        3559..3609
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             3659..4861
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             4885..5601
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      complement(5609..5661)
                     /label=L3S3P21
                     /note="L3S3P21"
                     /regulatory_class="terminator"