pTol2-HS-GT2MP-EGFP vector (V015805)

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LOCUS       62056_21230        5548 bp DNA     circular SYN 26-APR-2021
DEFINITION  Cloning vector pTol2-HS-GT2MP-EGFP, complete sequence.
ACCESSION   MW698954
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Lu C, Fan C, Wang J, Zhao Y.
  TITLE     Identification of muscle highly expression proximal cis-regulatory 
            element in zebrafish
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Lu C, Fan C, Wang J, Zhao Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2021) College of Fisheries and Life Science, 
            ShangHai Ocean University, No.999, Huchenghuan Rd, Nanhui, Shanghai 
            201306, China
REFERENCE   3  (bases 1 to 5548)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-MAR-2021) College of Fisheries and Life Science, ShangHai Ocean 
            University, No.999, Huchenghuan Rd, Nanhui, Shanghai 201306, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        411..515
                     /label=AmpR promoter
     misc_feature    700..1077
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      1546..2134
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2422..2443
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2458..2488
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2496..2512
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2520..2536
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2557..2575
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   2663..2868
                     /label=tol2-L
                     /note="tol2-L"
     misc_feature    2875..2946
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     CDS             3814..4527
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    4575..4799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4813..4904
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    4919..4990
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     repeat_region   5112..5261
                     /label=tol2-R
                     /note="tol2-R"
     promoter        complement(5292..5310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5320..5336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5478..5548
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"