pMT85-nanoLuc-Arg1 vector (V015800)

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LOCUS       V015800                 6373 bp    DNA     circular SYN 16-JAN-2020
DEFINITION  Exported.
ACCESSION   V015800
VERSION     V015800
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Pavlidis I, Spiller OB, Sammut Demarco G, MacPherson H, Howie SEM.,
            Norman JE, Stock SJ.
  TITLE     Cervical epithelial damage promotes Ureaplasma parvum ascending
            infection, intrauterine inflammation and preterm birth induction in
            mice
  JOURNAL   Nat Commun 11 (1), 199 (2020)
   PUBMED   31924800
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Spiller OB.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Department of Medical Microbiology, Division
            of Infection and Immunity, Cardiff University, School of Medicine,
            6th Floor University Hospital, Cardiff, Wales CF14 4XN, United
            Kingdom
REFERENCE   3  (bases 1 to 6373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SPAdes v. 3.9.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "199"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-AUG-2019) Department of Medical Microbiology, Division of
            Infection and Immunity, Cardiff University, School of Medicine, 6th
            Floor University Hospital, Cardiff, Wales CF14 4XN, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..6373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    144..160
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..184
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             424..936
                     /label="Nluc"
                     /note="NanoLuc(R) luciferase"
     CDS             1128..1640
                     /label="Nluc"
                     /note="NanoLuc(R) luciferase"
     CDS             2589..4025
                     /gene="aacA-aphD"
                     /label="Bifunctional AAC/APH"
                     /note="Bifunctional AAC/APH from Staphylococcus epidermidis
                     (strain ATCC 35984 / RP62A). Accession#: Q5HMP3"
     rep_origin      4120..4708
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"