pSfgfp-Sp vector (V015786)

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LOCUS       62056_19610        5332 bp DNA     circular SYN 22-DEC-2019
DEFINITION  Expression vector pSfgfp-Sp, complete sequence.
ACCESSION   MK948098
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Zhang J-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Center for Algal Biology and Applied 
            Research, Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 
            430072, 086
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2019) Center for Algal Biology and Applied Research, 
            Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 430072, 086"
FEATURES             Location/Qualifiers
     source          1..5332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2308..2334
                     /label=protein domain linker
                     /note="protein domain linker"
     CDS             2335..2352
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             2377..3084
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     misc_feature    3085..3114
                     /label=protein domain linker
                     /note="protein domain linker"
     CDS             3121..3144
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             3181..3204
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      3215..3262
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             3699..4487
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      4798..4816
                     /regulatory_class="terminator"
     primer_bind     complement(4938..4954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"