RS540-AAV-ErbB-RASER1C-OFPBidBH3 vector (V015784)

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LOCUS       62056_23260        7361 bp DNA     circular SYN 18-MAY-2019
DEFINITION  Cloning vector RS540-AAV-ErbB-RASER1C-OFPBidBH3, complete sequence.
ACCESSION   MK801287
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Chung HK, Zou X, Bajar BT, Brand VR, Huo Y, Alcudia JF, Ferrell JE 
            Jr., Lin MZ.
  TITLE     A compact synthetic pathway rewires cancer signaling to therapeutic 
            effector release
  JOURNAL   Science 364 (6439) (2019)
  PUBMED    31048459
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Chung HK, Lin MZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2019) Bioengineering, Stanford University, 269 
            Campus Drive, CCRS2100, Stanford, CA 94305, United States
REFERENCE   3  (bases 1 to 7361)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science 364
            (6439) (2019)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2019) Bioengineering, Stanford University, 269 Campus Drive,
            CCRS2100, Stanford, CA 94305, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=L-ITR
                     /note="L-ITR"
     misc_feature    137..142
                     /note="XbaI; Geneious type: restriction; XbaI"
     misc_feature    143..148
                     /label=XhoI
                     /note="XhoI"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     731..770
                     /label=p2006 Nhe1 TM for
                     /note="p2006 Nhe1 TM for"
     misc_feature    758..762
                     /label=nonstandard type: Editing History Replacement; C
     CDS             928..954
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     primer_bind     1068..1108
                     /label=p1940 TM Bsp1047I for
                     /note="p1940 TM Bsp1047I for"
     CDS             1180..1689
                     /label=CIBN
                     /note="N-terminal portion of the CIB1 transcription factor
                     from Arabidopsis thaliana"
     misc_feature    complement(1690..1719)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1853
     primer_bind     1705..1741
                     /label=p1727
                     /note="p1727"
     misc_feature    1723..2106
                     /label=nonstandard type: gBLOCK; HKC gBLOCK-5 VAV1
     misc_feature    1744..2076
                     /label=nonstandard type: SH2; VAV1
     misc_feature    2134..2151
                     /label=nonstandard type: polylinker; L6
     misc_feature    2188..2220
                     /label=nonstandard type: Substrate; substrate 4
     misc_feature    complement(2205..2245)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1942 sub4 
                     AscI
                     Bid rev
     misc_feature    2230..2316
                     /label=nonstandard type: misc; Bid
     CDS             2317..3045
                     /label=mCardinal
                     /note="far-red fluorescent protein derived from mNeptune,
                     with further red-shifted spectra"
     misc_feature    3052..3093
                     /label=nonstandard type: epitope; V5
     primer_bind     3052..3093
                     /label=p1291
                     /note="p1291"
     misc_feature    3103..3165
                     /note="T2A peptide from Thosea asigna virus capsid protein"
     misc_feature    3166..3723
                     /label=Shc-PTB
                     /note="Shc-PTB"
     misc_feature    complement(3192..3228)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1856 SmaI 
                     PTB
                     rev
     misc_feature    3529..3549
                     /label=HIF1a
                     /note="HIF1a"
     CDS             3727..3750
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    3787..4365
                     /label=nonstandard type: Protease; NS3Tprotease
     misc_feature    3946..3948
                     /label=nonstandard type: misc; T54
     primer_bind     4113..4145
                     /label=p1278
                     /note="p1278"
     misc_feature    complement(4131..4161)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1502 MreI 
                     rev
     misc_feature    complement(4344..4387)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse
     primer_bind     4366..4403
                     /label=p1992 EcoRI EcoRV polyA
                     /note="p1992 EcoRI EcoRV polyA"
     misc_feature    4369..4374
                     /label=EcoRV
                     /note="EcoRV"
     polyA_signal    4408..4615
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4616..4623
                     /label=NotI
                     /note="NotI"
     repeat_region   4624..4764
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4839..5294
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5576..5680
                     /label=AmpR promoter
     CDS             5681..6538
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6712..7300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"