pSL1180-EACMCV vector (V015782)

LOCUS       62056_19935        7668 bp DNA     circular SYN 18-NOV-2020
DEFINITION  Cloning vector pSL1180 EACMCV DNA-B 1.6mer, complete sequence.
ACCESSION   MT856192
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Deeply Sequenced Infectious Clones of Key Cassava Begomovirus 
            Isolates from Cameroon
  JOURNAL   Microbiol Resour Announc 9 (46), e00802-20 (2020)
  PUBMED    33184153
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2020) Ecology, Evolution, and Natural Resources, 
            Rutgers University, 14 College Farm Rd, New Brunswick, NJ 08901, USA
REFERENCE   3  (bases 1 to 7668)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc"; date: "2020"; volume: "9"; issue: "46"; pages: 
            "e00802-20"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-AUG-2020) Ecology, Evolution, and Natural Resources, Rutgers 
            University, 14 College Farm Rd, New Brunswick, NJ 08901, USA"
FEATURES             Location/Qualifiers
     source          1..7668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1047..1068
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1083..1113
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1121..1137
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1145..1161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1242..2924
                     /note="duplicated part of EACMCV DNA-B partial tandem
                     dimer, BclI to ClaI, inclusive, copy 1"
     misc_feature    1495..4226
                     /label=EACMCV DNA-B monomer unit, nick site to nick site
                     /note="EACMCV DNA-B monomer unit, nick site to nick site"
     gene            1801..2565
                     /gene="BV1"
                     /label=BV1
     CDS             1801..2565
                     /codon_start=1
                     /transl_table=11
                     /gene="BV1"
                     /product="NSP"
                     /label=BV1
                     /protein_id="QNR00640.1"
                     /translation="MYTTPRRGYRSAYRPRAIRRNYAKTPYKPVTKPPVNRRITFDSSK
                     QVYVRRSIEDVHSGANMQLANQGQQTSYVNFPSLGVDGNGGRCFDHIKLLNLRVSGTVS
                     VTQIGGDDPMGEQSLLRGIFLMAVIVDKRPFVPEGVNTLPTFKELFGEYDTVYGNPRLK
                     DNIRHRYRLLGTVKQYITTEEAHVQKPVNLRRKLSNARYPVWSSFKDLDASSTGGNYKN
                     VNKNAILVSYVWVSCERSKCDVYAQFVLNYVG"
     gene            complement(2706..3629)
                     /gene="BC1"
                     /label=BC1
     CDS             complement(2706..3629)
                     /codon_start=1
                     /transl_table=11
                     /gene="BC1"
                     /product="MP"
                     /label=BC1
                     /protein_id="QNR00641.1"
                     /translation="MDNQFTVTDNTYIHSKRTEYVLTNDAAPIHLQFPSSFEQATMRLK
                     GRCMKIDHIIIEYRNQVPFNATGSVIVEIRDNRVSLEDAAQAAFTFPIACNVDLHYFSS
                     TYFSISEPSPWSIMYRVEDSNVVEGVKFASIKAKLRLSSAKHSTDIRFKPPTINILSKG
                     YTKDCIDFWSVEKGETRRRLLNPTPTAHSQRPIAHRPITILPGETWATKSQLGLPCSSS
                     QRDIGCMRSQSMRIDPTTRPLDADNDSTEYPYQGLYRLSTPVLDPGDSVSQTPSDTVSR
                     KDLESLIETTINRCLIKTQCETPRQL"
     misc_feature    2925..3973
                     /note="unique part of EACMCV DNA-B partial tandem dimer,
                     ClaI to BclI, noninclusive"
     misc_feature    3974..5656
                     /note="duplicated part of EACMCV DNA-B partial tandem
                     dimer, BclI to ClaI, inclusive, copy 2"
     primer_bind     complement(5737..5753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5966..6421
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6703..6807
                     /label=AmpR promoter
     CDS             6808..7665
                     /label=AmpR
                     /note="beta-lactamase"