pETCC--TriF1 vector (V015767)

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LOCUS       V015767                 6169 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V015767
VERSION     V015767
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..6169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..88
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             120..142
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             161..178
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             188..205
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             209..1699
                     /gene="glpK"
                     /label="Glycerol kinase"
                     /note="Glycerol kinase from Thermococcus kodakarensis
                     (strain ATCC BAA-918 / JCM 12380 / KOD1). Accession#:
                     O93623"
     CDS             1757..2884
                     /gene="ackA"
                     /label="Acetate kinase"
                     /note="Acetate kinase from Mycolicibacterium smegmatis
                     (strain ATCC 700084 / mc(2)155). Accession#: A0QQK1"
     terminator      3881..3928
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(4294..4322)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        4435..4539
                     /label="AmpR promoter"
     CDS             4540..5397
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5571..6159
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"