pZS2-200 vector (V015760)

LOCUS       62056_23215        7658 bp DNA     circular SYN 02-MAY-2020
DEFINITION  Cloning vector pZS2-200, complete sequence.
ACCESSION   MN744968
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Gao C, Fernandez VI, Lee KS, Fenizia S, Pohnert G, Seymour JR, Raina
            J-B., Stocker R.
  TITLE     Single-cell bacterial transcription measurements reveal the 
            importance of dimethylsulfoniopropionate (DMSP) hotspots in ocean 
            sulfur cycling
  JOURNAL   Nat Commun 11 (1) (2020) In press
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2019) Biological Engineering, Massachusetts 
            Institute of Technology, 15 Vassar Street, Cambridge, MA 20139, USA
REFERENCE   3  (bases 1 to 7658)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            11 (1) (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2019) Biological Engineering, Massachusetts Institute of 
            Technology, 15 Vassar Street, Cambridge, MA 20139, USA"
FEATURES             Location/Qualifiers
     source          1..7658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..64
                     /label=RNAI
                     /note="RNAI"
                     /regulatory_class="terminator"
     regulatory      complement(65..238)
                     /label=TSAL2
                     /note="TSAL2"
                     /regulatory_class="terminator"
     CDS             complement(263..976)
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     protein_bind    complement(1032..1048)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1056..1086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1101..1122)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      1353..1852
                     /label=putative PdddW
                     /note="putative PdddW"
                     /regulatory_class="promoter"
     CDS             1889..2602
                     /label=mVenus
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     regulatory      2621..2743
                     /label=TR2-17
                     /note="TR2-17"
                     /regulatory_class="terminator"
     regulatory      2744..2820
                     /label=TL17
                     /note="TL17"
                     /regulatory_class="terminator"
     regulatory      2833..2885
                     /label=BS7
                     /note="BS7"
                     /regulatory_class="terminator"
     terminator      complement(2910..2937)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2953..3024)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3042..3749)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     RBS             complement(3758..3778)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(3808..4307)
                     /label=putative PdmdA
                     /note="putative PdmdA"
                     /regulatory_class="promoter"
     CDS             4538..5329
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5363..5457
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      5949..6171
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6219..7166
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"