pHpaC-C1 vector (V015755)

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LOCUS       V015755                 4514 bp    DNA     circular SYN 12-MAY-2020
DEFINITION  Exported.
ACCESSION   V015755
VERSION     V015755
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
   PUBMED   32341562
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 4514)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways,
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
FEATURES             Location/Qualifiers
     source          1..4514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1112
                     /gene="hpaC"
                     /label="4-hydroxyphenylacetate 3-monooxygenase reductase
                     component"
                     /note="4-hydroxyphenylacetate 3-monooxygenase reductase
                     component from Escherichia coli. Accession#: Q57501"
     misc_feature    1122..1178
                     /label="MCS"
                     /note="multiple cloning site"
     polyA_signal    1302..1423
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1430..1885)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1912..2016
                     /label="AmpR promoter"
     promoter        2018..2375
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2410..3201
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3436..3483
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3812..4400
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"