Cam_FruR_TAN_CelR_KSL vector (V015747)

MSDS

Basic Vector Information

Vector Name:: Cam_FruR_TAN_CelR_KSL

Antibiotic Resistance:: Chloramphenicol

Length:: 5302 bp

Type:: Cloning vector

Replication origin:: p15A ori

Source/Author:: Rondon RE, Groseclose TM, Short AE, Wilson CJ.

Cam_FruR_TAN_CelR_KSL vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Cam_FruR_TAN_CelR_KSL vector Sequence

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LOCUS       62056_456        5302 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector Cam_FruR_TAN_CelR_KSL, complete sequence.
ACCESSION   MN207956
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 5302)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(27..260)
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /label=rop protein
                     /note="maintains plasmids at low copy number"
                     /protein_id="QFU95595.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLQLFN
                     DPALNRRPGRICFRISAIHPLIITYSGVAPGV"
     CDS             complement(371..1384)
                     /codon_start=1
                     /transl_table=11
                     /product="CelR KSL"
                     /label=CelR KSL
                     /protein_id="QFU95596.1"
                     /translation="MKPVTLYDVAEYAGVSKSTVSLVVNQASHVSAKTREKVEAAIKEL
                     GYVPNRAARTLVTRRTDTVALVVSENNQKLFAEPFYAGIVLGVGVALSERGFQFVLATG
                     RSGIEHERLGGYLAGQHVDGVLLLSLHRDDPLPQMLDEAGVPYVYGGRPLGVPEEQVSY
                     VDIDNIGGGRQATQRLIETGHRRIATIAGPQDMVAGVERLQGYREALLAAGMEYDETLV
                     SYGDFTYDSGVAAMRELLDRAPDVDAVFAASDLMGLAALRVLRASGRRVPEDVAVVGYD
                     DSTVAEHAEPPMTSVNQPTELMGREMARLLVDRITGETTEPVRLVLETHLMVRESG"
     promoter        complement(1385..1462)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      complement(1477..1554)
                     /label=lacIq promoter
                     /note="lacIq promoter"
                     /regulatory_class="promoter"
     promoter        2163..2240
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2241..3245
                     /codon_start=1
                     /transl_table=11
                     /product="FruR TAN"
                     /label=FruR TAN
                     /protein_id="QFU95597.1"
                     /translation="MKPVTLYDVAEYAGVSTATVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKSSRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPD
                     NEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVVGAD
                     QDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYER
                     EAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDF
                     LQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS"
     protein_bind    3258..3279
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      3470..4014
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        4540..4642
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4643..5299
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"

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