pWRG7077---Makona-VP35 vector (V015743)

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LOCUS       V015743                 5249 bp    DNA     circular SYN 09-JAN-2021
DEFINITION  Exported.
ACCESSION   V015743
VERSION     V015743
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Beitzel B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2020) Center for Genome Sciences, US Army Medical
            Research Institute of Infectious Disease, 1425 Porter St, Ft
            Detrick, MD 21702, USA
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNAstar / SeqMan Ngen v. 12/2015 Sequencing
            Technology      :: PacBio
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-NOV-2020) Center for Genome Sciences, US Army Medical Research
            Institute of Infectious Disease, 1425 Porter St, Ft Detrick, MD
            21702, USA"
FEATURES             Location/Qualifiers
     source          1..5249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(302..1114)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     enhancer        1344..1724
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1725..1928
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2064..2889
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             2919..3938
                     /gene="VP35"
                     /label="Polymerase cofactor VP35"
                     /note="Polymerase cofactor VP35 from Zaire ebolavirus
                     (strain Kikwit-95). Accession#: Q6V1Q9"
     polyA_signal    3966..4190
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4418..5006)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"