pLL1228 vector (V015730)

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LOCUS       V015730                 8469 bp    DNA     circular SYN 05-OCT-2020
DEFINITION  Exported.
ACCESSION   V015730
VERSION     V015730
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Holwerda EK, Zhou J, Hon S, Stevenson D, Amador-Noguez D, Lynd LR,
            van Dijken JP.
  TITLE     Metabolic fluxes of nitrogen and pyrophosphate in chemostat cultures
            of Clostridium thermocellum and Thermoanaerobacterium
            saccharolyticum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Hon S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2020) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-APR-2020) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..573
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     gene            complement(840..1418)
                     /gene="tdk"
                     /label="tdk"
     CDS             complement(840..1418)
                     /codon_start=1
                     /transl_table=11
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /protein_id="QOE54900.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     regulatory      complement(1419..2039)
                     /label="C. thermocellum cbp promoter"
                     /note="C. thermocellum cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(2118..3119)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(3120..3574)
                     /direction=LEFT
                     /label="C. thermocellum origin of replication"
                     /note="C. thermocellum origin of replication"
     CDS             complement(3680..4537)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4538..4642)
                     /label="AmpR promoter"
     misc_feature    4732..5244
                     /label="upstream homologous recombination flank"
                     /note="upstream homologous recombination flank"
     misc_feature    5245..5736
                     /label="downstream homologous recombination flank"
                     /note="downstream homologous recombination flank"
     regulatory      5740..6314
                     /label="C. thermocellum gapdh promoter"
                     /note="C. thermocellum gapdh promoter"
                     /regulatory_class="promoter"
     CDS             6315..6962
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     gene            6983..7528
                     /gene="hpt"
                     /label="hpt"
     CDS             6983..7528
                     /codon_start=1
                     /transl_table=11
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /protein_id="QOE54904.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     misc_feature    7713..8226
                     /note="gene target internal homologous recombination
                     region"
     terminator      8259..8447
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"