Basic Vector Information
- Vector Name:
- pLBR2
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7329 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Li L.
pLBR2 vector Map
pLBR2 vector Sequence
LOCUS 62056_13715 7329 bp DNA circular SYN 13-MAY-2019 DEFINITION Cloning vector pLBR2, complete sequence. ACCESSION MK176921 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7329) AUTHORS Li L. TITLE Direct Submission JOURNAL Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 200032, China REFERENCE 2 (bases 1 to 7329) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 200032, China" FEATURES Location/Qualifiers source 1..7329 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(137..153) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(161..177) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(185..215) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(230..251) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(539..1127) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1635..2426) /label=NeoR/KanR /note="aminoglycoside phosphotransferase" oriT complement(3013..3122) /direction=LEFT /label=oriT /note="incP origin of transfer" CDS 3415..5199 /codon_start=1 /transl_table=11 /product="phiBT1 integrase" /label=phiBT1 integrase /protein_id="QCO69774.1" /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL LKEEDEASEATERELAAL" CDS complement(6860..6871) /label=Factor Xa site /note="Factor Xa recognition and cleavage site"
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