pcDNA3.1Zeo(-)-HBV1.1--D vector (V015721)

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LOCUS       62056_6110        8226 bp DNA     circular SYN 21-DEC-2019
DEFINITION  Cloning vector pcDNA3.1Zeo(-)-HBV1.1- D, complete sequence.
ACCESSION   MN645906
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of 
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg 
            69120, Deutschland
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2019) Molecular Virology, Department of Infectiology, 
            Heidelberg University Hospital, Inf344, Heidelberg 69120, 
            Deutschland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             918..1469
                     /codon_start=1
                     /transl_table=11
                     /product="core protein"
                     /label=core protein
                     /protein_id="QGX02070.1"
                     /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP
                     EHCSPHHTALRQAILCWGELMTLATWVGVNLEDPASRDLVVSYVNTNMGLKFRQLLWFH
                     ISCLTFGRETVIEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRGRSPRRRTPSP
                     RRRRSQSPRRRRSQSRESQC"
     CDS             2189..3031
                     /label=HBsAg (pre-S2/S)
                     /note="middle (pre-S2/S) envelope protein of hepatitis B
                     virus"
     CDS             3573..4037
                     /codon_start=1
                     /transl_table=11
                     /product="X protein"
                     /label=X protein
                     /protein_id="QGX02072.1"
                     /translation="MAARLCCQLDPARDVLCLRPVGAESCGRPFSGSLGTLSSPSPSAV
                     PTDHGAHLSLRGLPVCAFSSAGPCALRFTSARRMETTVNAHQILPKVLHKRTLGLSAMS
                     TTDLEAYFKDCLFKDWEELGEEIRLKVFVLGGCRHKLVCAPAPCNFFTSA"
     polyA_signal    4239..4463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4509..4937
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4951..5281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        5329..5376
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             5395..5766
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    5899..6032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6069..6085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6093..6109)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6117..6147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6162..6183)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6471..7059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7233..8090)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8091..8195)
                     /label=AmpR promoter