pUC19-ACMV vector (V015684)

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LOCUS       62056_21980        6876 bp DNA     circular SYN 18-NOV-2020
DEFINITION  Cloning vector pUC19 ACMV DNA-B 1.5mer, complete sequence.
ACCESSION   MT856194
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Deeply Sequenced Infectious Clones of Key Cassava Begomovirus 
            Isolates from Cameroon
  JOURNAL   Microbiol Resour Announc 9 (46), e00802-20 (2020)
  PUBMED    33184153
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2020) Ecology, Evolution, and Natural Resources, 
            Rutgers University, 14 College Farm Rd, New Brunswick, NJ 08901, USA
REFERENCE   3  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc"; date: "2020"; volume: "9"; issue: "46"; pages: 
            "e00802-20"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-AUG-2020) Ecology, Evolution, and Natural Resources, Rutgers 
            University, 14 College Farm Rd, New Brunswick, NJ 08901, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Benchling v. 2019-01-22
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1047..1068
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1083..1113
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1121..1137
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1145..1161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1174..2656
                     /note="duplicated part of ACMV DNA-B partial tandem dimer, 
                     HindIII to PstI inclusive, copy 1"
     misc_feature    2558..5282
                     /label=ACMV DNA-B monomer unit, nick site to nick site
                     /note="ACMV DNA-B monomer unit, nick site to nick site"
     misc_feature    2657..3898
                     /note="unique part of ACMV DNA-B partial tandem dimer, PstI
                     to HindIII, noninclusive"
     gene            2992..3762
                     /gene="BV1"
                     /label=BV1
     CDS             2992..3762
                     /codon_start=1
                     /transl_table=11
                     /gene="BV1"
                     /product="NSP"
                     /label=BV1
                     /protein_id="QNR00651.1"
                     /translation="MYSIRKQSRNLQRKYNSNTTNRYPIRRKYVAGHTRPCVRRRLSYE
                     PVERPLVHNVLCEKQHGDVFNLQQNTSYTSFVTYPARGSSGDGRSRDYIKLQSMSVSGV
                     IHAKADGNDDPMELSQVVNGVFVFSLIMDTKPYLPAGVQALPTFEELFGPYSACYVNLR
                     LLNNQQHRYRVLHSVKRFVSSSGDTKVSQFRFNKRLSTRRYTIWASFHDVDLVNAGGNY
                     RNISKNAILVSYAFVSEHAMSCKPFVQIETSYVG"
     gene            complement(3771..4667)
                     /gene="BC1"
                     /label=BC1
     CDS             complement(3771..4667)
                     /codon_start=1
                     /transl_table=11
                     /gene="BC1"
                     /product="MP"
                     /label=BC1
                     /protein_id="QNR00652.1"
                     /translation="MDTSVPVISSDYIHSARTEYKLTNDESPITLQFPSTLERTRVRIM
                     GKCMKVDHVVIEYRNQVPFNAQGSVIVTIRDTRLSDEQQDQAQFTFPIGCNVDLHYFSA
                     SYFSIDDNVPWQLLYKVEDSNVKNGITFAQIKAKLKLSAAKHSTDIKFKQPTIKILSKD
                     YGPDCVDFWSVGKPKPIRRLIQNEPGTDYDTGPKYRPITVQPGETWATKSTIGRSTSMR
                     YTGPQPIDIDASSSKQYASEAEFPLRGLHRLPEASLDPGDSVSQTQSMSKKDIESIIEQ
                     TVNKCLIAHRGSSHKDL"
     misc_feature    3899..5381
                     /note="duplicated part of ACMV DNA-B partial tandem dimer, 
                     HindIII to PstI inclusive, copy 2"
     primer_bind     complement(5421..5437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5911..6015
                     /label=AmpR promoter
     CDS             6016..6873
                     /label=AmpR
                     /note="beta-lactamase"