pTsodCPC9 vector (V015681)

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LOCUS       V015681                 6153 bp    DNA     circular SYN 08-JUL-2019
DEFINITION  Exported.
ACCESSION   V015681
VERSION     V015681
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Bosse JT, Li Y, Leanse LG, Zhou L, Chaudhuri RR, Peters SE, Wang J,
            Maglennon GA, Holden MTG., Maskell DJ, Tucker AW, Wren BW, Rycroft
            AN, Langford PR.
  TITLE     Rationally designed mariner vectors to allow functional genomic
            analysis of Actinobacillus pleuropneumoniae and other bacteria by
            transposon-directed insertion-site sequencing (TraDIS)
  JOURNAL   bioRxivorg (2019) In press
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Bosse JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2018) Medicine, Imperial College London, Norfolk
            Place, London W2 1PG, UK
REFERENCE   3  (bases 1 to 6153)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUL-2018) Medicine, Imperial College London, Norfolk Place,
            London W2 1PG, UK"
FEATURES             Location/Qualifiers
     source          1..6153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      4..279
                     /label="derived from Actinobacillus pleuropneumoniae sodC"
                     /note="derived from Actinobacillus pleuropneumoniae sodC"
                     /regulatory_class="promoter"
     CDS             289..1335
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /label="transposase"
                     /note="C9 mutant transposase from Himar1"
                     /protein_id="QDH76837.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDIKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     repeat_region   1437..1465
                     /label="Himar1 IR left"
                     /note="Himar1 IR left"
     misc_feature    1493..1504
                     /label="DNA uptake sequence (DUS) for Neisseria species"
                     /note="DNA uptake sequence (DUS) for Neisseria species"
     misc_feature    1503..1511
                     /note="DNA uptake signal sequence (USS) for Haemophilus
                     influenza"
     misc_feature    1512..1520
                     /note="DNA uptake signal sequence (USS) for Actinobacillus
                     pleuropneumoniae"
     CDS             1690..2337
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    2411..2419
                     /note="DNA uptake signal sequence (USS) for Actinobacillus
                     pleuropneumoniae"
     misc_feature    2419..2430
                     /label="DNA uptake sequence (DUS) for Neisseria species"
                     /note="DNA uptake sequence (DUS) for Neisseria species"
     misc_feature    2431..2439
                     /note="DNA uptake signal sequence (USS) for Haemophilus
                     influenza"
     repeat_region   2442..2470
                     /label="Himar1 IR right"
                     /note="Himar1 IR right"
     oriT            2688..2797
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             2830..3198
                     /label="traJ"
                     /note="oriT-recognizing protein"
     promoter        complement(3278..3296)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3314..3330)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3338..3354)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3362..3392)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3407..3428)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3716..4304)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4478..5335)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5336..5440)
                     /label="AmpR promoter"
     rep_origin      complement(5518..5973)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6114..6130
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6137..6153
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"