pFA-3xFLAG-MNase-CaARG4 vector (V015670)

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LOCUS       62056_10495        5232 bp DNA     circular SYN 30-SEP-2020
DEFINITION  Cloning vector pFA-3xFLAG-MNase-CaARG4, complete sequence.
ACCESSION   MT181238
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Tebbji F, Khemiri I, Sellam A.
  TITLE     High resolution genome-wide occupancy in Candida spp. using ChEC-seq
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Sellam A, Tebbji F, Khemiri I.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5232)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-2020) Microbiology, Infectious Disease "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..128
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     CDS             135..581
                     /label=MNase
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             1302..2708
                     /codon_start=1
                     /transl_table=11
                     /product="CaArg4"
                     /label=CaArg4
                     /note="CaArg4; argininosuccinate lyase"
                     /protein_id="QOE54958.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     promoter        complement(2870..2888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3146..3734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3908..4765)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4766..4870)
                     /label=AmpR promoter
     promoter        5216..5232
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"