L4440-2XproD-gtwy vector (V015665)

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LOCUS       62056_1390        4705 bp DNA     circular SYN 12-MAY-2021
DEFINITION  Cloning vector L4440-2XproD-gtwy, complete genome.
ACCESSION   MT333852
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Velasco L.
  TITLE     An efficient dsRNA constitutive expression system for bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Velasco L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2020) Plant Protection, Instituto Andaluz de 
            Investigacion y Formacion Agraria y Pesquera (IFAPA), Cortijo de la 
            Cruz s/n, Churriana, Malaga 29140, Spain
REFERENCE   3  (bases 1 to 4705)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2020) Plant Protection, Instituto Andaluz de Investigacion y
            Formacion Agraria y Pesquera (IFAPA), Cortijo de la Cruz s/n, 
            Churriana, Malaga 29140, Spain"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        587..605
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      616..775
                     /label=proD
                     /note="proD"
                     /regulatory_class="promoter"
     protein_bind    783..907
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        932..962
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     gene            1016..1696
                     /gene="cat"
                     /label=cat
     CDS             1016..1696
                     /codon_start=1
                     /transl_table=11
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CAT marker"
                     /protein_id="QUV72827.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNEYNSTAMSGRAGRKRV
                     DPAY"
     CDS             2015..2317
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2361..2485)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2500..2516)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2706..2724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2734..2750)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2892..3347
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3373..3477
                     /label=AmpR promoter
     CDS             3478..4335
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4509..4705
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"