Basic Vector Information
- Vector Name:
- pNC-BiFC-ED4
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 9870 bp
- Type:
- Plant binary expression vector
- Replication origin:
- pSa ori
- Host:
- Plants
- Source/Author:
- Yan P, Zeng Y, Shen W, Tuo D
- Promoter:
- CaMV 35S (enhanced)
pNC-BiFC-ED4 vector Vector Map
pNC-BiFC-ED4 vector Sequence
LOCUS 62056_17775 9870 bp DNA circular SYN 04-SEP-2019
DEFINITION Plant binary expression vector pNC-BiFC-ED4, complete sequence.
ACCESSION MK896893
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9870)
AUTHORS Yan P, Zeng Y, Shen W, Tuo D, Li X, Zhou P.
TITLE Direct Submission
JOURNAL Submitted (07-MAY-2019) Institute of Tropical Bioscience and
Biotechnology, Chinese Academy of Tropical Agriculture Sciences,
Xueyuan Road, Haikou, Hainan 571101, China
REFERENCE 2 (bases 1 to 9870)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(07-MAY-2019) Institute of Tropical Bioscience and Biotechnology,
Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road,
Haikou, Hainan 571101, China"
FEATURES Location/Qualifiers
source 1..9870
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 10..26
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 118..789
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
regulatory 799..929
/label=translational enhancer
/note="translational enhancer"
/regulatory_class="enhancer"
promoter 1112..1214
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 1245..1547
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS 1608..2126
/label=VN173
/note="N-terminal fragment of mVenus for use in bimolecular
fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
regulatory 2151..2361
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
CDS 2460..3113
/label=CmR
/note="chloramphenicol acetyltransferase"
regulatory 3223..3433
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
gene 3455..3649
/gene="CEYFP"
/label=CEYFP
CDS complement(3710..4012)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
promoter complement(4043..4145)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
regulatory 4328..4458
/label=translational enhancer
/note="translational enhancer"
/regulatory_class="enhancer"
promoter complement(4468..5139)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind complement(5231..5247)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5270..5286)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(5323..5341)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5362..5378)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5386..5402)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5410..5441)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5456..5477)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5716..5740
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(5831..6419)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6523..7335)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 7626..8061
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 8194..8216
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
terminator complement(8240..8491)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(8534..9325)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(9359..9538)
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 9808..9826
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 9852..9868
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
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