pFA-3xFLAG-MNase-CaURA3 vector (V015645)

Download GeneBank File(.gb)

LOCUS       V015645                 4623 bp    DNA     circular SYN 30-SEP-2020
DEFINITION  Exported.
ACCESSION   V015645
VERSION     V015645
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Tebbji F, Khemiri I, Sellam A.
  TITLE     High resolution genome-wide occupancy in Candida spp. using ChEC-seq
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Sellam A, Tebbji F, Khemiri I.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-MAR-2020) Microbiology, Infectious Disease "
FEATURES             Location/Qualifiers
     source          1..4623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..128
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     CDS             135..581
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             1247..2056
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2261..2279)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2537..3125)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3299..4156)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4157..4261)
                     /label="AmpR promoter"
     promoter        4607..4623
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"