Basic Vector Information
- Vector Name:
- pSEVAb157
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6103 bp
- Type:
- Cloning vector
- Replication origin:
- RSF1010 oriV
- Source/Author:
- Damalas SG, Batianis C, Martin-Pascual M, de Lorenzo V
- Promoter:
- lac UV5
pSEVAb157 vector Map
pSEVAb157 vector Sequence
LOCUS 62056_19530 6103 bp DNA circular SYN 27-FEB-2021 DEFINITION Cloning vector pSEVAb157, complete sequence. ACCESSION MW476668 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6103) AUTHORS Damalas SG, Batianis C, Martin-Pascual M, de Lorenzo V, Martins Dos Santos VAP. TITLE SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, BioBricks and Type IIS restriction enzyme standards JOURNAL Microb Biotechnol 13 (6), 1793-1806 (2020) PUBMED 32710525 REFERENCE 2 (bases 1 to 6103) AUTHORS Damalas SG, Batianis C, Martin Pascual M, de Lorenzo V, Martins dos Santos VAP. TITLE Direct Submission JOURNAL Submitted (14-JAN-2021) Laboratory of Systems and Synthetic Biology, Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE, The Netherlands REFERENCE 3 (bases 1 to 6103) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microb Biotechnol"; date: "2020"; volume: "13"; issue: "6"; pages: "1793-1806" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (14-JAN-2021) Laboratory of Systems and Synthetic Biology, Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE, The Netherlands" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6103 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..22 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with 'ATG'" regulatory 23..57 /label=promoter BBa_J23105 /note="promoter BBa_J23105" /regulatory_class="promoter" regulatory 66..77 /label=RBS.20 /note="RBS.20" /regulatory_class="ribosome_binding_site" CDS 84..797 /label=superfolder GFP /note="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" misc_feature 804..824 /label=BioBrick suffix /note="universal suffix for all parts" terminator 855..949 /label=lambda t0 terminator /note="transcription terminator from phage lambda" promoter 1022..1113 /label=AmpR promoter CDS 1114..1971 /label=AmpR /note="beta-lactamase" terminator 1978..2017 /label=fd terminator /note="central terminator from bacteriophage fd (Otsuka and Kunisawa, 1982)" oriT 2164..2272 /label=oriT /note="incP origin of transfer" CDS complement(2286..3134) /label=RSF1010 RepC /note="replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" CDS complement(3124..3960) /label=RSF1010 RepA /note="replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" CDS complement(4474..5442) /label=RSF1010 RepB /note="replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" promoter complement(5466..5496) /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" rep_origin 5552..5946 /label=RSF1010 oriV /note="replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)" terminator complement(5978..6064) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene"
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