pVEcLa4 vector (V015639)

LOCUS       V015639                 6217 bp    DNA     circular SYN 31-JUL-2019
DEFINITION  Exported.
ACCESSION   V015639
VERSION     V015639
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Vezina B, Moore RJ.
  TITLE     Bioinformatic analysis and development of molecular tools for
            broiler chickens live Lactobacillis vaccine vector candidate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Vezina B, Moore RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2019) Microbiology, Monash University, Monash
            University, Clayton Campus, Wellington Rd, Clayton, VIC 3800,
            Australia
REFERENCE   3  (bases 1 to 6217)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JAN-2019) Microbiology, Monash University, Monash University,
            Clayton Campus, Wellington Rd, Clayton, VIC 3800, Australia"
FEATURES             Location/Qualifiers
     source          1..6217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             101..814
                     /codon_start=1
                     /transl_table=11
                     /product="Rep pLa3_4"
                     /label="Rep pLa3_4"
                     /note="Rep protein"
                     /protein_id="QDQ71306.1"
                     /translation="MPEKKKDNRARTWTFIVYPESVPNNWRELLDSYHIPWVESPLHDK
                     DVNPDGTIKKPHWHVILMFDGKKSYEQIKEITDSLNAPVPQKTANPKGLVRYLIHMDNP
                     EKYQYKKEDIVCHSGAEVEQYFQLSSASRLQILKEMIIFIKDSRFENYLDFLAYCIENG
                     EDEWLDIAVNHNTLALSKVIDSVYQKNHPKTPQTDYKQTVVAKAKEMARQGTSQRVIAD
                     TLGISPATVNRYLKK"
     CDS             1587..1901
                     /codon_start=1
                     /transl_table=11
                     /product="ORF-2"
                     /label="ORF-2"
                     /protein_id="QDQ71307.1"
                     /translation="MLFLTNFRQKARLNSRYPTSCVLWRGPTWFLLTSPWSDSKGKVRR
                     SFFGSFFVASNENKNSFCKNDLGFLNFKNYFCENKNKKARSKNTVRFFIFDFGFEENIF
                     "
     CDS             complement(1957..2115)
                     /codon_start=1
                     /transl_table=11
                     /product="ORF-3"
                     /label="ORF-3"
                     /protein_id="QDQ71308.1"
                     /translation="MIYFLWLSNQSLSKNKKIPTKSGAVTAISKEFIPSPPFQLAKGHS
                     NLSIISR"
     CDS             2361..2981
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     promoter        complement(3342..3360)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3378..3394)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3402..3418)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3426..3456)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3471..3492)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3780..4368)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4542..5399)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5400..5504)
                     /label="AmpR promoter"
     rep_origin      complement(5582..6037)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6178..6194
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6201..6217
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"