pTOX9-tse2-tsPurple vector (V015634)

LOCUS       V015634                 6826 bp    DNA     circular SYN 30-JUN-2019
DEFINITION  Exported.
ACCESSION   V015634
VERSION     V015634
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Lazarus JE, Warr AR, Kuehl CJ, Giorgio RT, Davis BM, Waldor MK.
  TITLE     A New Suite of Allelic Exchange Vectors for the Scarless
            Modification of Proteobacterial Genomes
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
   PUBMED   31201277
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Lazarus JE, Warr AR, Kuehl CJ, Giorgio RT, Davis BM, Waldor MK.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2019) Medicine, Massachusetts General Hospital, 55
            Fruit St, Jackson 5, Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 6826)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAY-2019) Medicine, Massachusetts General Hospital, 55 Fruit St,
            Jackson 5, Boston, MA 02114, USA"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..6826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      42..75
                     /label="strong forward terminator"
                     /note="strong forward terminator"
                     /regulatory_class="terminator"
     promoter        326..428
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             429..1085
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      1189..1275
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1367..1394
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1597..1715
                     /label="rhaB promoter"
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with
                     D-glucose in the presence of RhaR and RhaS (Giacalone et
                     al., 2006)"
     CDS             1756..2229
                     /gene="tse2"
                     /label="Toxin Tse2"
                     /note="Toxin Tse2 from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: Q9I0E0"
     terminator      2292..2538
                     /label="lambda tL3 terminator"
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    complement(2574..2590)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(2625..3013)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3634..4002)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(4035..4144)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     regulatory      5057..5074
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /regulatory_class="promoter"
     regulatory      5075..5156
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /regulatory_class="promoter"
     CDS             5184..6017
                     /label="rhaS"
                     /note="positive regulator of the rhaB promoter"
     gene            6044..6090
                     /gene="apFAB46"
                     /label="apFAB46"
     misc_feature    6044..6090
                     /gene="apFAB46"
                     /label="similar to apFAB46"
                     /note="similar to apFAB46"
     gene            6099..6110
                     /gene="B0034"
                     /label="B0034"
     misc_feature    6099..6110
                     /gene="B0034"
                     /label="similar to B0034"
                     /note="similar to B0034"
     CDS             6117..6800
                     /label="tsPurple"
                     /note="tsPurple is a basic (constitutively fluorescent)
                     fluorescent protein published in 2018, derived from
                     synthetic construct."