pGED vector (V015633)

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LOCUS       V015633                 6938 bp    DNA     circular SYN 14-OCT-2020
DEFINITION  Exported.
ACCESSION   V015633
VERSION     V015633
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Satanowski A, Dronsella B, Noor E, Voegeli B, He H, Wichmann P, Erb
            TJ, Lindner SN.
  TITLE     Awakening a latent carbon fixation cycle in Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Satanowski A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2020) Synthetic and Systems Metabolism Group, Max
            Planck Institute for Molecular Plant Physiology, Am Muehlenberg 1,
            Potsdam, Brandenburg 12163, Germany
REFERENCE   3  (bases 1 to 6938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2020) Synthetic and Systems Metabolism Group, Max Planck
            Institute for Molecular Plant Physiology, Am Muehlenberg 1, Potsdam,
            Brandenburg 12163, Germany"
FEATURES             Location/Qualifiers
     source          1..6938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..39
                     /label="pgi20"
                     /note="pgi20"
                     /regulatory_class="promoter"
     regulatory      67..93
                     /label="spacer"
                     /note="spacer"
                     /regulatory_class="other"
     regulatory      94..109
                     /label="RBS 'C'"
                     /note="RBS 'C'"
                     /regulatory_class="ribosome_binding_site"
     CDS             113..130
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             131..1531
                     /gene="gnd"
                     /label="6-phosphogluconate dehydrogenase, decarboxylating"
                     /note="6-phosphogluconate dehydrogenase, decarboxylating
                     from Escherichia coli (strain K12). Accession#: P00350"
     regulatory      1547..1573
                     /label="spacer"
                     /note="spacer"
                     /regulatory_class="other"
     regulatory      1574..1589
                     /label="RBS 'C'"
                     /note="RBS 'C'"
                     /regulatory_class="ribosome_binding_site"
     CDS             1593..1610
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1611..2246
                     /gene="eda"
                     /label="KHG/KDPG aldolase"
                     /note="KHG/KDPG aldolase from Escherichia coli (strain
                     K12). Accession#: P0A955"
     regulatory      2262..2288
                     /label="spacer"
                     /note="spacer"
                     /regulatory_class="other"
     regulatory      2289..2304
                     /label="RBS 'C'"
                     /note="RBS 'C'"
                     /regulatory_class="ribosome_binding_site"
     CDS             2308..2325
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2326..4131
                     /gene="edd"
                     /label="Phosphogluconate dehydratase"
                     /note="Phosphogluconate dehydratase from Escherichia coli
                     (strain K12). Accession#: P0ADF6"
     promoter        4166..4269
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4270..4926
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      5020..5106
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(5275..5820)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(5934..6028)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(6051..6839)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(6840..6931)
                     /label="AmpR promoter"