pAH63-AF_THS vector (V015627)

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LOCUS       62056_2940        6842 bp DNA     circular SYN 22-MAY-2021
DEFINITION  Integration vector pAH63-AF_THS, complete sequence.
ACCESSION   MT424825
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Foster AD, Chung C-W., Hann MM, Simpson GL, Tavassoli A.
  TITLE     Development of a fluorescent three-hybrid system for the 
            identification of protein-protein associators
  JOURNAL   Pept Sci (Hoboken) 113 (1), e24178 (2021)
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Foster AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2020) Chemistry, University of Southampton, 
            Building 27, University Road, Southampton SO17 1BJ, United Kingdom
REFERENCE   3  (bases 1 to 6842)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pept Sci 
            (Hoboken)"; date: "2021"; volume: "113"; issue: "1"; pages: "e24178"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2020) Chemistry, University of Southampton, Building 27, 
            University Road, Southampton SO17 1BJ, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..6842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      561..623
                     /label=P22/434
                     /note="P22/434"
                     /regulatory_class="promoter"
     CDS             624..1244
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             1245..1277
                     /label=ssrA tag
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
     regulatory      1287..1330
                     /regulatory_class="terminator"
     promoter        1440..1513
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     CDS             1543..2970
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     CDS             2992..3651
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     CDS             3657..3668
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     CDS             3734..4522
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      4583..4829
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    complement(4953..5186)
                     /label=lambda attP
                     /note="integrase from phage lambda"
     rep_origin      complement(5253..5641)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(5731..6522)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      6736..6822
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"