pLC1 vector (V015617)

LOCUS       62056_13730        5948 bp DNA     circular SYN 12-MAY-2019
DEFINITION  Cloning vector pLC1, complete sequence.
ACCESSION   MK159855
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..5948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     509..525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(575..671)
                     /regulatory_class="promoter"
     primer_bind     complement(692..708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(716..732)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(740..770)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(785..806)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1094..1682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1935..2735
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(3172..3540)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(3573..3682)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3920..3931)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    4002..4101
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             4111..5925
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"