pUCTABciVI vector (V015616)

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LOCUS       62056_22365        3247 bp DNA     circular SYN 30-NOV-2019
DEFINITION  Cloning vector pUCTABciVI, complete sequence.
ACCESSION   MK318074
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ghosh U, Basu U.
  TITLE     A pUC19 DERIVED PLASMID THAT GENERATES VECTOR FOR TA CLONING UPON 
            BciVI DIGESTION
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Ghosh U, Basu U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2018) Moleculer Biology and Biotechnology, 
            University of Kalyani, Kalyani, Kalyani, West Bengal 741235, India
REFERENCE   3  (bases 1 to 3247)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2018) Moleculer Biology and Biotechnology, University of 
            Kalyani, Kalyani, Kalyani, West Bengal 741235, India"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    287..292
                     /label=NcoI restriction site
                     /note="NcoI restriction site"
     primer_bind     complement(605..621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(629..645)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(653..683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(698..719)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1007..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1768..2625)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2626..2730)
                     /label=AmpR promoter
     misc_feature    3060..3065
                     /label=NarI restriction site
                     /note="NarI restriction site"
     primer_bind     3204..3220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3221..3226
                     /label=EcoRI restriction site
                     /note="EcoRI restriction site"
     misc_feature    3227..3232
                     /label=SacI restriction site
                     /note="SacI restriction site"
     misc_feature    3242..3247
                     /label=BamHI restriction site
                     /note="BamHI restriction site"