pTrcHisA-GFP(teLAA)-(teF)MCP vector (V015607)

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LOCUS       62056_21385        5958 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pTrcHisA-GFP(teLAA)-(teF)MCP, complete sequence.
ACCESSION   MK258730
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (05-DEC-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     CDS             383..406
                     /label=minicistron
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial 
                     expression of a downstream cistron (Schoner, 1997)"
     CDS             425..442
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             482..505
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             521..1237
                     /label=EGFP
                     /note="enhanced GFP"
     CDS             1244..1264
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1268..1300
                     /label=ssrA tag
                     /note="ssrA tag"
     regulatory      1310..1321
                     /label=b0034 RBS
                     /note="b0034 RBS"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1337..1354
                     /label=TEVp cleave site
                     /note="TEVp cleave site"
     misc_feature    1355..1369
                     /label=F degron
                     /note="F degron"
     CDS             1388..2080
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      2311..2397
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2489..2516
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2536..2627
                     /label=AmpR promoter
     CDS             2628..3485
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3659..4247
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4433..4573)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4759..4836
                     /label=lacI promoter
     CDS             4837..5916
                     /label=lacI
                     /note="lac repressor"