pUC57-Kan-mcr-8 vector (V015600)

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LOCUS       62056_22190        4587 bp DNA     circular SYN 13-JUN-2020
DEFINITION  Expression vector pUC57-Kan-mcr-8 DNA, complete seuence.
ACCESSION   LC548752
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of 
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho, 
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        466..494
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            742..2439
                     /gene="mcr-8"
                     /label=mcr-8
     CDS             742..2439
                     /codon_start=1
                     /transl_table=11
                     /gene="mcr-8"
                     /product="lipid A phosphoethanolamine transferase MCR-8"
                     /label=mcr-8
                     /protein_id="BCG50375.1"
                     /translation="MFKYLLSFKLNPVQRTWAAAFFFTTIGNIALWQTLWINVDVHNIH
                     NLLFFASLPIFLFCFLSILLTPVMVIPYLCRPLLVVLILISACCSYFMMKYNILIDRSM
                     VQNFFETNQAELTSYLSVPFLSTLFLLGIVPAIILALPSTDNKRGAFRIELWWLAHICI
                     AVVLLAMVTMVFYKDYASLIRNNMQIKDQALPFNFVRNTNGYLKRKYQASSTILQSVGE
                     DAVRPIYSNAPPKLVVVVVGETARAQNFQLNGYSRVTNPYLSRRHDVISFKNVSSCGTA
                     TAISLPCMFSRMSRNEYNEVRAASEENLLDILKRTGVEVLWRNNNNGGCKGICKRVPTD
                     DMPAMKVIGECVNKDGTCFDEVLLNQLSSRINAMQGDALIVLHQMGSHGPTYFERYPST
                     SKVFSPTCDSNLIEKCSNKELVNTYDNTLVYTDRMLSKTIELLQRYSGMRDVAMIYLSD
                     HGESLGESGIYLHGTPYIIAPNEQTHIPMFMWFSSSFAQHSKLNLECLTGNADKQYSHD
                     NFYHSILGLFNVKTSVYKPELDMFTLCRQSDHTPLSSAVVREKTDGNG"
     primer_bind     complement(2497..2513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2521..2537)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2545..2575)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2590..2611)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2899..3487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3668..4474)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"