pECpOE-GFP-N-N5L vector (V015594)

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LOCUS       62056_8795        6753 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Expression vector pECpOE-GFP-N-N5L, complete sequence.
ACCESSION   MN989859
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2222..3071
                     /label=Candida albicans TDH3 promoter
                     /note="Candida albicans TDH3 promoter"
                     /regulatory_class="promoter"
     protein_bind    3078..3102
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3103..3816
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     protein_bind    complement(3820..3844)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3865..4105
                     /label=Saccharomyces cerevisiae URA3 terminator
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     misc_feature    4126..4499
                     /note="recombination target; Candida parapsilosis NEUT5L 
                     sequence upstream"
     misc_feature    4500..4505
                     /label=StuI site for plasmid linearization
                     /note="StuI site for plasmid linearization"
     misc_feature    4506..4889
                     /note="recombination target; Candida parapsilosis NEUT5L 
                     sequence downstream"
     terminator      complement(4916..5113)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5131..5694)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     promoter        complement(5714..6057)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(6114..6132)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6139..6155)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(6296..6751)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"